N. Jamil ¹, R. Che Man ¹, S. Md Shaarani ¹, S. Z. Sulaiman ¹, S. K. Abdul Mudalip ¹, Z. I. Mohd Arshad ¹

Affiliations
1 Faculty of Chemical Engineering and Natural Resources, Universiti Malaysia Pahang, 26300 Gambang, MY

Abstract

Objectives: Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme, which undergoes cyclization reaction to converts starch into Cyclodextrin (CD). Due to their potential properties, CDs had been discovered to have numerous application in food industries, pharmaceutical, agriculture and also environmental engineering. To improve the production of cyclodextrin (CD) in the future, characterization of α-CGTase on the effect of operating conditions was investigated. Methods/Statistical Analysis: The α-CGTase from Bacillus licheniformis was characterized by examining their cyclization activity. The enzymatic activity of the enzyme towards the temperature and pH was determined by methyl orange method. Findings: The enzyme was estimated to be 70 kDa by the gel electrophoresis. The cyclization activity of α-CGTase was highest at a temperature of 40°C and pH 6.0. The α-CGTase enzyme was able to extend its thermostability up to 60°C with pH stability between pH 6.0 and pH 8.0. Application/Improvements: High production of CD is expected to obtain by using the optimal conditions which in turn may be beneficial for the industrial purpose.

Keywords

Cyclodextrin Glucanotransferase, Bacillus licheniformis, Characterization, Cyclization Activity, Cyclodextrin.

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N. Masngut ¹, S. Manap ¹, R. Che Man ¹, S. Shaarani ¹

Affiliations
1 Faculty of Chemical Engineering and Natural Resources, Universiti Malaysia Pahang, Gambang - 26300, Pahang, MY

Abstract

Background/Objectives: The purpose of this study is to isolate the potential bacteria for the production of industrial enzyme cellulose and xylanase for waste degradation. Methods/Statistical Analysis: The bacteria were isolated from three different sources (landfill surface, 10 cm depth soil and moist soil) collected from Sungai Ikan Landfill, Kuala Terengganu, Malaysia using serial dilution and spread plate technique. Primarily screening for the enzyme production was carried out. The bacteria isolates which shows a good clearing zone diameter in ranged 2.6-8.8 cm were selected for further quantitative screening for cellulase and xylanase production by performed submerged fermentation using Carboxymethyl Cellulose (CMC) and xylan as a substrate. Findings: A total of 130 potential bacteria were isolated on Nutrient Agar (NA). Only 50 and 30 of the isolates exhibited cellulase and xylanase, respectively, by showing clear zone on CMC and Xylan agar plates after stained with iodine dye. From the observation of the quantitative screening of enzyme activities, B6.2 isolate exhibited the highest cellulase activity (endoglucanase19.797 U/m Land FPase 7.384 U/mL) and M29 isolate exhibited the highest activity of xylanase (5399 U/mL). Overall, all isolates showed good cellulase and xylanse activity ranged between 0.491-19.797 U/mL and 2075-5399 U/mL, respectively. Application/Improvements: For future study, the enzyme produce will be used to degrade waste into fermentable sugars which used as a feedstock for bioethanol production.

Keywords

Cellulose-Degrading Bacteria, Cellulase, Landfill Soil, Xylanase, Xylan-Degrading Bacteria.

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